52128 Histopathology

Course Description

This course describe Introduction, fixation, decalcification, tissueprocessing, section cutting, staining, and special staining.

Learning Objective

  • At the end of this course the student is expected to:
  • 1. Recognize and describe the regulatory and safety procedures for specimen reception, identification and in non-surgical cut-up.
  • 2. Recognize and describe what constitutes good fixation of tissue, be able to recognize correctly fixed tissue and the different fixation programs required for different types of tissue
  • 3. Describe and recognize steps involved in routine and specialized tissue processing as well as the requirements for good tissue processing.
  • 4. Recognize and understand the principles of microtomy and the different types of microtomes and their specific purposes.
  • 5. Describe mordant dyes, solvent dyes, polychromatic dyes, neutral stains, amphoteric dyes and metachromatic dyes.
  • 6. Define, describe, and give examples of physical, chemical staining and metallic impregnation.
  • 7. Recognize and describe the histological demonstration of microorganisms, the cell wall of gram negative and gram positive bacteria and the theory and purpose of Gram stain, Ziehl-Neelson technique and Grocott’s methanamine silver as well as the appropriate control tissues.
  • 8. Describe the staining method for special stains, including Perl’s, Masson Fontana and von Kossa method. Describe normal versus pathological calcification.
  • 9. State the purpose of carbohydrate special stains including Periodic acid Schiff with and without diastase and Alcian blue stain.
  • 10. Describe the methods for the connective tissue stains including Van Gieson technique, Masson’s trichrome, Verhoeff’s, Gomori’s aldehyde fuchsin and Gordon & Sweets method
  • 11. Explain the importance of quality control and health and safety procedures in Histopathology and Cytopathology laboratories



Books for this Course

  • Bancroft, J.D. and Stevens, A.: theory and practice of histological techniques ed.3,

Times Offered

  • february 2016
  • June 2016

Course Prerequisite