Course DescriptionThis course describe Introduction, fixation, decalcification, tissueprocessing, section cutting, staining, and special staining.
- At the end of this course the student is expected to:
- 1. Recognize and describe the regulatory and safety procedures for specimen reception, identification and in non-surgical cut-up.
- 2. Recognize and describe what constitutes good fixation of tissue, be able to recognize correctly fixed tissue and the different fixation programs required for different types of tissue
- 3. Describe and recognize steps involved in routine and specialized tissue processing as well as the requirements for good tissue processing.
- 4. Recognize and understand the principles of microtomy and the different types of microtomes and their specific purposes.
- 5. Describe mordant dyes, solvent dyes, polychromatic dyes, neutral stains, amphoteric dyes and metachromatic dyes.
- 6. Define, describe, and give examples of physical, chemical staining and metallic impregnation.
- 7. Recognize and describe the histological demonstration of microorganisms, the cell wall of gram negative and gram positive bacteria and the theory and purpose of Gram stain, Ziehl-Neelson technique and Grocott’s methanamine silver as well as the appropriate control tissues.
- 8. Describe the staining method for special stains, including Perl’s, Masson Fontana and von Kossa method. Describe normal versus pathological calcification.
- 9. State the purpose of carbohydrate special stains including Periodic acid Schiff with and without diastase and Alcian blue stain.
- 10. Describe the methods for the connective tissue stains including Van Gieson technique, Masson’s trichrome, Verhoeff’s, Gomori’s aldehyde fuchsin and Gordon & Sweets method
- 11. Explain the importance of quality control and health and safety procedures in Histopathology and Cytopathology laboratories
Books for this Course
- Bancroft, J.D. and Stevens, A.: theory and practice of histological techniques ed.3,
- february 2016
- June 2016